Hard ticks are vectors of numerous pathogens responsible for human and veterinary diseases. In particular the Ixodes genus is distributed world-wide and comprises several species that transmit the Lyme borreliosis agent (Borellia sp.). To better understand evolutionary patterns in this genus (for example how genes related to blood-feeding evolved, and how fast did they change over evolutionary time), an accurate phylogeny of the whole group is needed (Chmelar, 2016). Using high output sequencing technology (RNA-Seq), we investigated phylogenetic relationships in the group of hard ticks. Transcriptomes from 9 species in the Ixodes genus were sequenced and were combined with data obtained from Genbank for two other Ixodes species as well as 9 non-Ixodes tick species. We reconstructed transcriptomes for each species (20 in total), predicted their coding sequences and performed sequence comparisons among them in order to identify Single copy orthologs (SCO). Using Maximum-likelihood and Bayesian framework, we combined a supertree and a supermatrix approach to obtain a reliable scenario of the hard tick phylogeny (Gonzalez, 2015). If major nodes of the tree were well resolved, parts of the phylogenetic tree still remain difficult to resolve. Overall, our results confrmed previous work on ticks phylogeny (Burger, 2012) and bringed new insight, in particular for the debated "ricinus complex" species (Xu, 2003). This work provides a better frame to study the evolutionnay history of ticks, and will facilitate further analyses of phylogeny and gene evolutionary patterns.