Characterization of the biosynthesis of saponins during seed development in peas (<em>Pisum sativum</em>) - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2019

Characterization of the biosynthesis of saponins during seed development in peas (Pisum sativum)

Abstract

The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart a bitter taste to the final food product. Bitter flavours are currently either removed by energyrequiring physico-chemical treatments or masked by additives. We are in the process of identifying and characterizing the genes involved in saponin biosynthesis during pea seed development, with the objective of identifying mutants in which seed saponins no longer accumulate. To do this we have applied a saponin extraction protocol to follow the biosynthesis of these compounds during the development of pea seeds, and have identified mutants in a Pea TILLING population (1) that correspond to key steps in the saponin biosynthetic pathway. A mutation in a previously identified β-Amyrin Synthase gene (2), which is highly expressed in maturing pea seeds, reduced mature seed saponin content by 97% (3).

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Dates and versions

hal-02737647 , version 1 (02-06-2020)

Identifiers

  • HAL Id : hal-02737647 , version 1
  • PRODINRA : 485157

Cite

Richard Thompson, Julie Marais, Ludivine Lebeigle, Christine Le Signor, Marie-Aleth Lacaille-Dubois, et al.. Characterization of the biosynthesis of saponins during seed development in peas (Pisum sativum). ILS3 2019, Third International Legume Society Conference. Legumes for human and planet health, International Legume Society (ILS). INT., May 2019, Poznan, Poland. ⟨hal-02737647⟩
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