Influence of pH and hydraulic retention time on hydrogen and ethanol co-production by dark fermentation in a CSTR with glycerol as substrate
Résumé
The effect of pH and HRT on hydrogen and ethanol production thought dark fermentation from pure glycerol was studied. Most of the results found in literature belong to batch cultures. In this study aerobic sludge was used as inoculum in a continuous stirred tank reactor (CSTR). A full factorial design with two variables (pH and HRT) and two levels was performed. Results indicate that these variables highly influence, both the hydrogen yield on glycerol (from 0.04 to 0.41 mole H2/mole gly) and ethanol yield on glycerol (from 0.005 to 0.052 mole ethanol/mole gly), obtaining differences up to an order of magnitude between them in steady state. Also, CE-SSCP fingerprinting analysis was performed, and showed considerable changes in the microbial diversity between different steady states studied, confirming the high effect of these variables over the process.
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