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Poster communications
Trims and antiviral immunity in fish: A FINTRIM inducing constitutive IFN signaling is expressed at surfaces exposed to pathogens
Abstract : The tripartite - motif - protein (TRIM) family includes many key components of the antiviral arsenal expressed in mammals; antiviral TRIMs mediate intrinsic viral restriction at diverse points of the viral cycle, or positively regulate innate immune signaling pathways. The TRIM family is ancient and has been greatly diversified in vertebrates and especially in fish. Our previous survey of fish TRIM genes in fish identifie d subsets with different evolutionary dynamics, with several subsets highly diversified li ke th e finTRIMs. We show here that a zebrafish finTRIM gene constitutively expressed in the gills, skin and pharynx, encodes a protein that strongly up - regulates the type I interferon (IFN) pathway. While it is not IFN - inducible, its in vivo expression in gills of healthy fish correlates with that of type I IFN. In vitro , overexpression of this finTRIM induce s IFN and IFN - stimulated gene expression, and affords protection against different enveloped and non - enveloped RNA viruses. This antiviral activity is IFN - dependent, and is abolished by a dominant negative IRF3 mutant. Our work indicates that TRIM proteins contribute to the establishment of antiviral immunity , possibly by permanent type I IFN stimulation in exposed tissues . Hence TRIMs might create a loc al anti - viral environment at sites exposed to pathogens, a mechanism participating to the regionalization of immunity. Our data also reveal that TRIMs were involved in antiviral immunity before the divergence between bony fish and tetrapods, early in Verte brate evolution.
https://hal.inrae.fr/hal-02742871
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Wednesday, June 3, 2020 - 4:58:47 AM
Last modification on : Thursday, April 7, 2022 - 10:10:30 AM
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Wednesday, June 3, 2020 - 4:58:47 AM
Last modification on : Thursday, April 7, 2022 - 10:10:30 AM
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