When poultry are slaughtered, they undergo official veterinary ante and post-mortem controls, covering all important aspects for protection of public health, animal health and welfare. Today, controls’ organization must be based on an assessment of animal health risks and food safety1,2. The active participation of operators is envisaged for these sanitary controls under the responsibility and supervision of veterinarians. Nevertheless scientific methods are needed to implement new approaches to sanitary controls on poultry in holdings and slaughterhouses to ensure they cover all their objectives. Multidisciplinary working groups composed of members from the national competent veterinary authority, epidemiologists, hygienists, veterinarians, and operators’ representatives were created. These groups defined the goals to reach for public health, animal health and welfare purposes. For each of them, the tasks were characterized and indicators of their achievement proposed. Five tasks were described: i. sanitary auditing on holding of provenance by a veterinarian; ii. “descending” food chain information analysis before each flock arrival at slaughterhouse; iii. ante-mortem and iv. post-mortem examinations; v. standardized “ascending food chain information”. Sanitary auditing on holding by a veterinarian and standardized “ascending food chain information” are the two examples described here. For sanitary auditing on holding, a questionnaire and a guide aiming at clarifying what to observe and how to answer the questions were designed. The questionnaire consists of a brief description of the holding followed by questions covering biosecurity and public health topics. For “ascending food chain information”, ante and post mortem examination data of different batches are standardized and sent to the holding, in order to be interpreted by the veterinarian attending the holding. The visible anomalies are synthesized as epidemiological, organic and etiological data. Anomalies’ detectability in the conditions of processing plant and sanitary risk were both considered in the data analysis. Descriptive factsheets of anomalies were then designed for a national reference frame in slaughterhouses. Finally, our work allowed the proposal of news tools relevant to the veterinary public health monitoring throughout the poultry chain. The proposed procedures, as well as the effective participation of different actors, were assessed on a sample of slaughterhouses and holdings.