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Identification de mécanismes de résistance non liée à la cible grâce à la transcriptomique

Abstract : We have witnessed a dramatic increase in the number of weeds showing herbicide resistance during the past three decades. The potentially most dangerous mechanisms of resistance endows Non-Target-Site based Resistance (NTSR): NTSR is polygenic, unpredictable and can confer cross-resistance to herbicides with different modes of action. High throughput sequencing methods, like RNA sequencing (RNA-seq), are particularly effective to identify genes differentially expressed between two experimental modalities. Therefore, RNA sequencing was implemented to identify genes involved in NTSR to clodinafop (an ACCase inhibitor) in black-grass. Black-grass transcriptome was sequenced from 4 sensitive plants and 4 clodinafop-resistant plants from the same F1 population, collected at different time-points before and after herbicide treatment. A black-grass transcriptome database was established that contains qualitative data (sequence of each gene and associated protein) and quantitative data (gene expression level of every gene during the time-course, in sensitive and in resistant plants). The expression of candidate NTSR genes was subsequently verified by quantitative RT-PCR.
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https://hal.inrae.fr/hal-02747026
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Submitted on : Wednesday, June 3, 2020 - 11:14:17 AM
Last modification on : Monday, July 4, 2022 - 8:58:10 AM

Identifiers

  • HAL Id : hal-02747026, version 1
  • PRODINRA : 261555

Citation

Jeanne Gardin, Sébastien Carrère, Jerome Gouzy, B. Couloume, C. Engel, et al.. Identification de mécanismes de résistance non liée à la cible grâce à la transcriptomique. 22. Conférence du Columa, Journées internationales sur la lutte contre les mauvaises herbes, Association Française de Protection des Plantes (AFPP). FRA., Dec 2013, Dijon, France. 45 p. ⟨hal-02747026⟩

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