ABA-independent SnRK2 kinases are involved in plant response to salinity
Résumé
Sucrose non-fermenting 1-Related Protein Kinases type 2 family (known as SnRK2) are important group of kina- ses present in all tested plant species. It is known that all SnRK2 (excluding SnRK2.9) are activated in response to salinity and hyperosmolarity in Arabidopsis thaliana protoplasts after short time of treatment. In plants, they are in- volved in the early responses to drought and salinity. Recently published data indicate that SnRK2.4 plays important role in the maintenance of root system architecture during salt stress and in response to cadmium treatment. It was also suggested that in roots facing cadmium treatment, SnRK2.4 controls ROS accumulation leading to alteration of stress tolerance. Here, we studied the involvement of ABA-independent SnRK2 kinases in ROS accumulation and scavenging during salt stress in A. thaliana leaves by investigating the level of glutathione and hydrogen peroxide in insertion mutants of SnRK2.4 and SnRK2.10. We also established the role of kinases studied as regulators of plant tolerance to salt stress by testing chlorophyll degradation in KO lines.