Insyght proposes a new way to explore the landscape of conserved and idiosyncratic genomic regions across multiple genomes and their rearrangements throughout evolution. Its unique display consists of a symbolic representation tightly integrated with a proportional view. The symbols highlight a region of interest and provide legibility while the proportional view simultaneously allows grasping genomic locations and complex rearrangements scattered across the genomes and occurring at different scales. A second type of display is dedicated to the analysis of the presence, absence, or multiple copies of a given set of homologs. A functionality based on filters has been implemented to facilitate the retrieval of genes of interest and allow the formulation of relevant biological questions, such as finding niche-specific or core genome genes that match a few particular functions or biological processes. Our public dataset currently consists of 389 prokaryotes genomes. Alternatively, a virtual machine can be downloaded and installed locally to visualize private data. It contains a pre-installed version of the pipeline, database and visualisation tool. Insyght is suitable for a variety of analyses: genome-wide inference of gene function, detection of evolutionary events, phylogenetic profiling and investigation of the core genome or niche-specific genes.