Accéder directement au contenu Accéder directement à la navigation
Communication dans un congrès

Disturbance frequency affects morphology and community composition of a biofilm

Abstract : Biofilms are spatially heterogeneous at the scale of micrometers in terms of microbial composition, physiological state, and biofilm morphology. It is often neglected that maturing biofilms in a flow field also develop a heterogeneous spatial structure on the order of millimetres and centimetres. At this larger scale, the heterogeneity of biofilms is likely an important parameter affecting substrate diffusion into the biofilm and consequently influences the availability of substrate to the microbial community. How disturbance frequency, in our case the interval of monochloramine addition, influences the evolution of biofilm morphology and bacterial community structure on a larger scale is the objective of our study. Multispecies biofilms are grown on defined synthetic substrate in three 5-liters bubble column reactors. While one reactor serves as control without biocide addition, two reactors receive a biocide pulses daily and every other day. In each reactor, over a period of 2 months, biofilm is sampled on 133 removable coupons for the acquisition of images with a size of nearly 4 cm2 and for DNA extractions. DNA and images allow us to perform community fingerprinting using single strand conformation polymorphisms and automated image analysis of biofilm morphology. We showed in initial experiments that a minimum image size is necessary to capture heterogeneities in biofilm morphology relevant for mass transfer and thus substrate availability. This size is larger than typical dimensions of images acquired with e.g. confocal microscopes. We introduce a novel strategy for large-scale biofilm imaging to relate biofilm inhabitants to macroscopic heterogeneities on the order of millimetres and centimetres. In three parallel reactors, we relate microbial diversity to disturbance frequency and biofilm morphology.
Type de document :
Communication dans un congrès
Liste complète des métadonnées

https://hal.inrae.fr/hal-02753429
Déposant : Migration Prodinra <>
Soumis le : mercredi 3 juin 2020 - 19:32:31
Dernière modification le : jeudi 5 novembre 2020 - 09:08:38

Identifiants

  • HAL Id : hal-02753429, version 1
  • PRODINRA : 270083

Citation

Kim Milferstedt, Jean-Jacques Godon, Renaud Escudie, Nicolas Bernet. Disturbance frequency affects morphology and community composition of a biofilm. 13. International Symposium on Microbial Ecology ISME 13 - Stewards of a changing planet, International Society for Microbial Ecology (ISME). INT., Aug 2010, Seattle, United States. ⟨hal-02753429⟩

Partager

Métriques

Consultations de la notice

9