Introduction The replication duration represents a crucial step in the life cycle of a virus which influences many parameters of virus biology such as the kinetics of its accumulation in the infected cells and tissues, in the development of the infection within the host and beyond, epidemiology and viral evolution. Despite its central role during viral infection, quantitative parameters of the replication cycle such as its duration are largely unknown, especially for plant viruses. This lack of information conduct us to design an experimental system for measuring the replication cycle duration of Cauliflower mosaic virus (CaMV). Objective Determination of the replication cycle duration of CaMV in Arabidopsis thalianaprotoplasts. We define the duration of replication as the time required for a viral particle, to replicate its genome and form new viral particles after its entry in the host cell....