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Communication dans un congrès

Identification of molecular markers linked to ms8 gene in sweet pepper (Capsicum annuum L.)

Abstract : Nuclear ms8 gene is a single recessive gene which can be used to develop the male sterility system, applicable in sweet pepper hybrid seed production. Such a nuclear male sterility system would be more effective if molecular markers of ms8 gene were available. Thus we have made an attempt to find molecular markers linked to ms8 locus. A male sterile plant of line 320 was crossed with Elf variety in order to develop F2 mapping population 320 x Elf. Line 320 is a male sterile Bulgarian-type red fruited line and Elf is a blocky-type yellow fruited sweet pepper variety. RAPD and DArT technologies combined with BSA (Bulked Segregant Analysis) were used to identify molecular markers of ms8 gene. Seven RAPD markers linked to rns8 gene were identified and converted to dominant SCAR markers. Two developed SCAR markers were segregating in the doubled haploid population from the F1 (Yolo Wonder x Perennial) and were mapped on the lower arm of the pepper chromosome P4 COSII markers available for lower arm of chromosome P4 were tested in 320 x Elf population and two markers were segregating showing rather weak linkage with ms8 locus. DArT BSA analysis resulted in the identification of seven DArT markers potentially linked to rns8 locus. The applicability of the identified markers in the breeding programmes will be subject to further studies.
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Communication dans un congrès
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https://hal.inrae.fr/hal-02757171
Déposant : Migration Prodinra <>
Soumis le : mercredi 3 juin 2020 - 23:57:48
Dernière modification le : mercredi 1 juillet 2020 - 16:48:05

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  • HAL Id : hal-02757171, version 1
  • PRODINRA : 38158

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G. Bartoszewski, I. Stepien, P. Gawronski, Cesary Waszczak, Véronique Lefebvre, et al.. Identification of molecular markers linked to ms8 gene in sweet pepper (Capsicum annuum L.). 14. Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Aug 2010, Valencia, Spain. ⟨hal-02757171⟩

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