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Real-time RT-PCR and cDNA macroarray to study the impact of the genetic polymorphism at the alphaS1-casein locus on the expression of genes in the goat mammary gland during lactation

Abstract : Milk fat has a large effect on nutritional, technological and sensorial properties of milk products. The milk fat content and composition are modulated by genetics and nutritional factors and imply a large number of enzymes. The regulation of their gene expression in the mammary gland still needs to be clarified. An association between the extensive polymorphism at the alpha(sl)-ca-casein (alpha(sl)-Cas) locus and both the lipid content and the characteristics of this fraction in caprine milk has been demonstrated. In order to decipher the mechanism responsible for this impact, a quantification of the transcripts of four lipogenic key enzymes (acetyl-CoA carboxylase, fatty acid synthase, lipoprotein lipase and stearoyl-CoA desaturase) was performed using real-time RT-PCR, suggesting an absence of association between the alpha(sl)-Cas genotype and expression variability of the studied genes. This approach has been completed by a more global analysis using a first generation of ruminant macroarray gathering 400 gene probes. The comparison of the expression profiles of lactating goat alpha(sl)-Cas A/A (strong allele) and F/F (defective allele) mammary gland allowed to confirm the expected variability in the expression of known genes (such as those encoding the alpha(sl)-casein) in ruminant mammary tissues as welt as to identify up- and down-regulated genes. A second generation of ruminant cDNA macroarray extended to a few thousands of genes is currently in progress and will be applied to study different factors such as the nutritional regulation of gene expression in the mammary gland.
Keywords : rt-pcr adnc
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https://hal.inrae.fr/hal-02764107
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Submitted on : Thursday, June 4, 2020 - 7:42:52 AM
Last modification on : Tuesday, July 20, 2021 - 4:03:32 AM

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Christine Leroux, Fabienne Le Provost, F. Petit, Laurence Bernard, Yves Chilliard, et al.. Real-time RT-PCR and cDNA macroarray to study the impact of the genetic polymorphism at the alphaS1-casein locus on the expression of genes in the goat mammary gland during lactation. INRA Meeting on Genomic, Proteomic and Lactation, Nov 2002, Jouy en Josas, France. ⟨10.1051/rnd:2003032⟩. ⟨hal-02764107⟩

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