INRA BrACySol Biological Resource Center (BRC) conserves a large Brassica collection which includes around 900 B. oleracea populations and 3600 rapeseed lines. Cabbage populations have been collected in farms before the massive use of hybrids. These accessions are conserved as frozen seeds. Seed batches are renewed every 10 – 15 years according to their germination rate. Project ‘SecureBracysol’, funded by GIS IBiSA, aimed at improving the management of this collection by dealing with two issues. On the one hand, in order to identify putative duplicates, a molecular fingerprinting of all accessions was performed. For each population, two bulks of 20 seeds have been genotyped by BioGEVES with 9 SSR markers chosen for their allelic diversity and genome coverage. Genetic distances using Dice dissimilarity index have been calculated to compare the two bulks of each population and to compare populations between each other. The two bulks gave similar profiles for 55% of the populations, indicating an inter-bulk diversity. Some populations that could not be distinguished need to be further analysed with additional SSR markers. On the other hand, genetic drift from one multiplication cycle was evaluated by analysing 9 accessions chosen to represent 3 cultigroups (cauliflower, cabbage, kale). For each accession, 90 plants from each of two generations were genotyped by BioGEVES with 17 SSR chosen using same criteria as above. Several genetic diversity indices were calculated and showed that two generations did not differ significantly for each analysed accession. These results validate the multiplication process which is in use.