We will use DUV DISCO beamline to perform measurement of fluorescence anisotropy using TMA-DPH membrane staining. With fluorescence anisotropy data on yeast strains with contrasted omega 3 content, we will explore the link between omega 3 content and membrane fluidity. This work will provide fundamental data on the physiology of Debaryomyces species which are key yeasts for food and non-food biotechnologies. Membrane fluidity data obtained on DISCO beamline, combine with a set of phylogeny, genomic and biochemical existing results will allow major advances on omega 3 metabolism in Debaryomyces species and will contribute to our largest project on the study of the Saccharomycotina subphylum.