Storage of <em>Trichogramma chilonis</em> (Hymenoptera :Trichogrammatidae) to improve IPM strategies against the sugarcane stem borer, <em>Chilo sacchariphagus</em> (Lepidoptera : Crambidae) - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Conference Papers Year : 2014

Storage of Trichogramma chilonis (Hymenoptera :Trichogrammatidae) to improve IPM strategies against the sugarcane stem borer, Chilo sacchariphagus (Lepidoptera : Crambidae)

Elisabeth Tabone
  • Function : Author
Etty Colombel
  • Function : Author
Maurane Buradino
Hong Do Thi Khanh
  • Function : Author


The stem borer Chilo sacchariphagus is a key pest of sugarcane in Reunion Island and South-East Asia. Since 2002, an IPM strategy associating inundative release of an egg parasitoid, Trichogramma chilonis and conservation of a predatory ant, Pheidole megacephala, has been proposed. According to field experiments in Reunion Island, this reduced up to 50% of damage with financial gains ranging from 800 to 1800 dollars per ha. To developp IPM strategies on a large scale, reducing the costs of beneficials and releasing field labour is necessary. In this context, cold storage is a key step to the development of the sugarcane borer biocontrol, by improving production and release management.Thanks to a funding from the French Ministry of Agriculture (CASDAR), laboratory tests on cold storage of T. chilonis were first conducted, followed by field experiments on the performance of stored parasitoids. Quality control after storage was performed several time (after mass production, before and after transport, after field release) in laboratory at 25+/-1°C, RH 75+/-5% and 16L:8D to evaluate emergence rate of stored insects, fecundity during 7 days and survival at 7th day of T.chilonis females. For the first time, a long-term cold storage of T.chilonis was obtained without loss of physiological performance (good emergence, fecundity, survival during 7 days). Results suggested a quiescence state because no loss was observed due to storage interruption. Moreover, the same efficacy of cold stored Trichogramma compared to non-stored individuals was obtained in the field (exprimental sites in Reunion Island). These important results make possible a large scale application of IPM strategies in sugarcane production
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Dates and versions

hal-02797786 , version 1 (05-06-2020)


  • HAL Id : hal-02797786 , version 1
  • PRODINRA : 308686


Elisabeth Tabone, Etty Colombel, Maurane Buradino, Hong Do Thi Khanh. Storage of Trichogramma chilonis (Hymenoptera :Trichogrammatidae) to improve IPM strategies against the sugarcane stem borer, Chilo sacchariphagus (Lepidoptera : Crambidae). Insectinov - Insectes : une filière d'avenir pour les biotechnologies, Adebiotech. FRA., Dec 2014, Paris, France. ⟨hal-02797786⟩
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