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The porcine socs and their involvement in the response to two strains of influenza a virus

Abstract : The Suppressors Of Cytokine Signaling (SOCS) and the Cytokine-Inducible SH2 domain containing protein (CISH) are a family of eight proteins which have emerged as key regulators of the immune system. Little is known about tissue expression of SOCS and data in pigs are still scarce. In order to go further in the study of porcine SOCS, additional data are needed. CISH and SOCS transcript expressions along with the stability of some reference genes were assessed in various tissues. Then, we characterized the immune response of Newborn Pig Trachea cells (NPTr) and Porcine Alveolar Macrophages (PAMs) to a German strain (H3N2) and a Canadian strain (H1N1) of swine influenza and the potential role of SOCS proteins in this response. Among SOCS, only SOCS1 appears to be over-expressed. Using Precision Cut Lung Slices (PCLS) we characterized the interaction of H3N2 and H1N1 with the respiratory mucosa in the first hours of infection using different multiplicity of infection. By immunofluorescence the presence of the virus in the epithelial cells was confirmed and the establishment of a type I interferon response along with the subsequent mRNA expression of antiviral genes such as Mx1, Mx2, OAS1, RNAseL and PKR were observed in PAMs and NPTr cells. Furthermore, the influence of H3N2 on cellular signalisation was analysed at early points of infection in NPTr cells. We observed a precocious activation of JAK/STAT and MAPK pathways such as p38 and ERK 1-2. Then, kinome and RTqPCR analyses of the impact of H1N1 infection of NPTr cells in the presence of an inhibitor of SOCS1/SOCS3 (pJAK2) is in progress. Together, our data show the establishment of a broad antiviral response in NPTr cells, PAMs, and PCLS against H3N2 and H1N1, and strongly suggest a regulation by SOCS1 requiring further studies.
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Déposant : Migration Prodinra <>
Soumis le : samedi 6 juin 2020 - 01:55:21
Dernière modification le : jeudi 16 juillet 2020 - 15:36:06


  • HAL Id : hal-02806268, version 1
  • PRODINRA : 273897



Mario Delgado Ortega, Sandrine Melo, Joëlle Dupont, Darsaniya Punyadarsaniya, Georg Herrler, et al.. The porcine socs and their involvement in the response to two strains of influenza a virus. 10. International Veterinary Immunology Symposium (IVIS), Aug 2013, Milan, Italy. 254 p., 2013. ⟨hal-02806268⟩



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