Primordial germ cells (PGCs) are the precursors of germ cell lineage. Located in the epiblast at stage X embryo (EG&K), the PGC translocate anteriorly to the germinal crescent and migrate through the blood vascular system to the germinal ridges within 48-56 hours of development, where they become the gonadal germ cells. We aim to compare the in vitro long-term cultured PGCs derived from the chicken blood of a 2 days old embryo (stages 14 to 17 HH) with the long-term cultured gonocytes taken from male gonads of a 5-6 days old embryo (stages 29 to 30 HH) and determined their basic characters. The results of immunostaining demonstrated that anti-SSEA-1, anti-EMA-1, anti-CVH, anti-integrin β1 and anti-CEACAM, together with PAS stain, bound specifically to chicken PGCs and GGCs. The reactivity of chicken embryonic germ cells obtained from the two different sources to the specific markers used in this study was not altered through the culture. In conclusion, the morphological analysis specific for chicken PGCs and GGCs presented in this study, will further contribute to quick and reliable characterization of these cell types in vitro .