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Poster

Exploiting transcript profiling data to decipher rice – Magnaporthe grisea compatibility mechanisms

Abstract : Whereas the molecular events associated with disease resistance are largely studied, those occurring in susceptible plants are poorly known, especially in Monocots. We chose the rice/Magnaporthe grisea system to explore the molecular modifications that are induced in susceptible plant tissues following pathogen attack. A truly compatible interaction (between O. sativa cultivar Nipponbare and M. grisea isolate FR13) at time points where infection occurs without visible symptoms was studied. Changes in rice transcript levels at 3 and 4 days post inoculation were measured using Affymetrix chips. In addition to the up-regulation of a large number of defence-related genes, this analysis revealed new features indicating extensive reprogramming of host gene expression. Microarray expression data were confirmed for 35/38 genes using quantitative RT-PCR, thus demonstrating the robustness of these transcript profiling experiments. This comprehensive survey of global gene expression in response to colonization by a virulent fungal isolate constitutes an important resource that should help us to further our understanding of the rice blast disease process.
Mots-clés : RELATION HOTE-PARASITE
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Poster
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https://hal.inrae.fr/hal-02814665
Déposant : Migration Prodinra <>
Soumis le : samedi 6 juin 2020 - 12:20:18
Dernière modification le : jeudi 27 août 2020 - 10:02:03

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  • HAL Id : hal-02814665, version 1
  • PRODINRA : 10176

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Judith Hirsch, Virginie Pacaly, Didier Tharreau, Jean-Loup Notteghem, Jean-Benoit Morel. Exploiting transcript profiling data to decipher rice – Magnaporthe grisea compatibility mechanisms. 4. International Rice Blast Conference, Oct 2007, Changsha, China. n.p., 2007. ⟨hal-02814665⟩

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