Assessment of uranium and selenium speciation in human and bacterial biological models to probe changes in their structural environment - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Communication Dans Un Congrès Année : 2009

Assessment of uranium and selenium speciation in human and bacterial biological models to probe changes in their structural environment

Résumé

This study illustrates the potential of physicochemical techniques to speciate uranium (U) and selenium (Se) in biological samples. Speciation, defined here as the study of structural environment, of both toxic elements, was characterized at several levels in biological media and directly in human cells or bacteria once the metal(loid)s were internalized. External speciation that is extracellular speciation in culture media was predicted by thermodynamic equilibrium computer modelling using the JChess software and validated by spectroscopic measurements (XANES and EXAFS). Internal speciation that is intracellular speciation in eukaryotic and prokaryotic cells was studied in vitro with a soil bacterium Cupriavidus metallidurans CH34 and ROS 17/2.8 osteoblasts, human cells responsible for bone formation. XANES, EXAFS, HPLC-ICP-MS and SDS-PAGE coupled to particle induced X-ray emission (PIXE) permitted the identification and quantification of complexes formed with organic or inorganic molecules and/or larger proteins.
Fichier non déposé

Dates et versions

hal-02816702 , version 1 (06-06-2020)

Identifiants

  • HAL Id : hal-02816702 , version 1
  • PRODINRA : 157986

Citer

Laure Avoscan, Sarah Milgram, Guillaume Unterreiner, Richard Collins, H. Khodja, et al.. Assessment of uranium and selenium speciation in human and bacterial biological models to probe changes in their structural environment. 3.Workshop on Chemical Speciation, Jan 2008, Montpellier, France. ⟨hal-02816702⟩
23 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More