Background: Human activities introduce organic and metallic contaminants in the cropped soils that can alter their functioning. Environmental risk assessment needs the development of tools to quantify the ecotoxicity of the contaminants. Among soil organisms, filamentous fungi produce enzymes involved in the biogeochemical cycles. These enzymes may be used as indicators of fungal exposure to contaminants in soils. Aims: The present study aims to establish how the developing phases of the fungus Trametes versicolor modulate the expression of extracellular enzymes after exposure to copper. For that purpose, it is necessary to monitor the activities of selected enzymes during the fungal growing and the stationary phases without metal contamination, then in the presence of the metal. Methods: Hydrolase activities (namely urease, arylsulfatase, acidic and alkaline phosphatases, b-D-glucosidase and N-acetyl-b-glucosaminidas) as well as oxidase activities (laccase, manganese and lignin-peroxidases) were measured during two weeks in liquid cultures of Trametes versicolor. Copper was added either during the growing or the stationary phases. Results: The results show that urease, b-D-glucosidase, acidic phosphatase and N-acetyl-b-glucosaminidase are mainly expressed during the growing phase of the fungus without metal spiking. By contrast, activities of the oxidases and b- galactosidase are increased during the stationary phase. Copper modify the expression of the enzymes. As a general way, hydrolase activities are decreased whereas oxidase ones are strongly increased. The sensitivity of enzymatic responses to copper depends on the development of the fungus and the type of secreted enzyme. Conclusion: The exposure of Trametes versicolor to copper modulate the expression of its extracellular enzymes, whatever its developing phase. It is now necessary to identify enzymatic profiles that could be used as tools for metal ecotoxicity assessment.