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Pré-publication, Document de travail

A plasma membrane nanoplatform ensures signal specificity during osmotic signaling in plants

Résumé : In the course of their growth and development plants have to constantly perceive and react to their environment. This is achieved in cells, by the coordination of complex combinatorial signaling networks. However, how signal integration and specificity are achieved in this context is unknown. With a focus on the hyperosmotic stimulus, we use live super-resolution light imaging methods to demonstrate that a Rho GTPase, Rho-of-Plant 6 (ROP6), forms stimuli-dependant nanodomains within the PM. These nanodomains are necessary and sufficient to transduce production of reactive oxygen species (ROS),that act as secondary messengers and trigger several plant adaptive responses to osmotic constraints. Furthermore, ROP6 activation triggers the nanoclustering of two NADPH oxidases that subsequently generates ROS. ROP6 nanoclustering is also needed for cell surface auxin signaling, but short-time auxin treatment does not induce ROS accumulation. We show that auxin-induced ROP6 nanodomains, unlike osmotically-driven ROP6 clusters, do not recruit the NADPH oxidase, RBOHD. Together, our results suggest that Rho GTPase nano-partitioning at the PM ensure signal specificity downstream of independent stimuli.
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https://hal.inrae.fr/hal-02873744
Déposant : Chantal Baracco <>
Soumis le : jeudi 18 juin 2020 - 15:02:27
Dernière modification le : jeudi 2 juillet 2020 - 14:23:38

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Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale 4.0 International License

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M. Smokvarska, C. Francis, M.P. Platre, J.B. Fiche, C. Alcon, et al.. A plasma membrane nanoplatform ensures signal specificity during osmotic signaling in plants. 2020. ⟨hal-02873744⟩

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