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Curvature and Torsion of Protein Main Chain as Local Order Parameters of Protein Unfolding

Abstract : Thermal protein unfolding resembles a global (two-state) phase transition. At the local scale, protein unfolding is, however, heterogeneous and probe dependent. Here, we consider local order parameters defined by the local curvature and torsion of the protein main chain. Because chemical shift (CS) measured by NMR spectroscopy is extremely sensitive to the local atomic environment, CS has served as a local probe of thermal unfolding of proteins by varying the position of the atomic isotope along the amino-acid sequence. The variation of the CS of each C(alpha) atom along the sequence as a function of the temperature defines a local heat-induced denaturation curve. We demonstrate that these local heat-induced denaturation curves mirror the local protein nativeness defined by the free-energy landscape of the local curvature and torsion of the protein main chain described by the C(alpha)-C(alpha) virtual bonds. Comparison between molecular dynamics simulations and CS data of the gpW protein demonstrates that some local native states defined by the local curvature and torsion of the main chain, mainly located in secondary structures, are coupled to each other whereas others, mainly located in flexible protein segments, are not. Consequently, CS of some residues are faithful reporters of global protein unfolding, with heat-induced denaturation curves similar to the average global one, whereas other residues remain silent about the protein unfolded state. For these latter, the local deformation of the protein main chain, characterized by its local curvature and torsion, is not cooperatively coupled to global unfolding.
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Article dans une revue
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https://hal.inrae.fr/hal-02886011
Déposant : Sabine Julien <>
Soumis le : mercredi 1 juillet 2020 - 10:48:22
Dernière modification le : jeudi 2 juillet 2020 - 03:42:41

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Paul Grassein, Patrice Delarue, Adrien Nicolaï, Fabrice Neiers, Harold Scheraga, et al.. Curvature and Torsion of Protein Main Chain as Local Order Parameters of Protein Unfolding. Journal of Physical Chemistry B, American Chemical Society, 2020, 124 (22), pp.4391-4398. ⟨10.1021/acs.jpcb.0c01230⟩. ⟨hal-02886011⟩

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