Effect of dilution rate, cellobiose and ammonium availabilities on Clostridium cellulolyticum sporulation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Applied Microbiology and Biotechnology Year : 1999

Effect of dilution rate, cellobiose and ammonium availabilities on Clostridium cellulolyticum sporulation

Abstract

The nutritional and physiological factors affecting sporulation of Clostridium cellulolyticum were studied using steady-state continuous cultures grown in both complex and synthetic media. Under cellobiose limitation, the probability that cells will sporulate appears to be directly related to the growth rate. In complex medium, the highest percentage of sporulation was 20% at a dilution rate of 0.015 h A1 whereas in synthetic medium it was 10% at 0.035 h A1. In both media, when the dilution rate was either higher or lower the percentage of sporulation decreased by between 2% and 4%. At low dilution rates, endospore formation was repressed under cellobiose-sucient concentrations, suggesting catabolite repression by cellobiose. Furthermore , the concentration of ammonium was important in determining the percentage of sporulation, as ammo-nium limitation induced extensive sporulation at low growth rates even in an excess of cellobiose. The sporulation process is not triggered when cells are cel-lobiose-exhausted both in complex and synthetic media. These data suggest that, in C. cellulolyticum, an exoge-nous supply of carbon is required throughout the sporulation process. In the experimental conditions used in this work, no relationship between glycogen accumulation or glycogen mobilization and endospore formation was detected in C. cellulolyticum.

Dates and versions

hal-02910824 , version 1 (03-08-2020)

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Cite

Sophie Payot, Emmanuel Guedon, Mickaël Desvaux, Éric Gelhaye, E. Petitdemange. Effect of dilution rate, cellobiose and ammonium availabilities on Clostridium cellulolyticum sporulation. Applied Microbiology and Biotechnology, 1999, 52 (5), pp.670-674. ⟨10.1007/s002530051577⟩. ⟨hal-02910824⟩

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