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Surface Rheological Properties of Native and S-Ovalbumin Are Correlated with the Development of an Intermolecular β-Sheet Network at the Air−Water Interface

Abstract : Polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS), ellipsometry, and shearelastic constant measurements were used to study the adsorption and the behavior of ovalbumin andS-ovalbumin at the air-water interface at different values of the subphase pH. Native and S-ovalbuminexhibited similar behaviors, with a maximum plateau value of the shear elastic constant near the isoelectricpH of the protein. However, higher surface concentration values were reached with S-ovalbumin in lownet charge conditions, which suggest adsorption of aggregates or multilayer adsorption. For both proteins,the statistical analysis of PM-IRRAS spectra demonstrated that the aging of the interfacial film and theincrease of the shear elastic constant were correlated with a significant increase in the relative contributionof intermolecular‚-sheets in the amide I band with time. This increase was significantly faster at low pHvalues. At the same pH value and age of the interface, the relative contribution of intermolecular‚-sheetswas significantly higher for S-ovalbumin
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https://hal.inrae.fr/hal-02913795
Contributor : Stéphane Pezennec <>
Submitted on : Monday, August 10, 2020 - 3:42:13 PM
Last modification on : Thursday, June 10, 2021 - 4:16:09 PM

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Anne Renault, Stéphane Pezennec, Fabien Gauthier, Véronique Vié, Bernard Desbat. Surface Rheological Properties of Native and S-Ovalbumin Are Correlated with the Development of an Intermolecular β-Sheet Network at the Air−Water Interface. Langmuir, American Chemical Society, 2002, 18 (18), pp.6887-6895. ⟨10.1021/la0257586⟩. ⟨hal-02913795⟩

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