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Bovine Leukemia Virus-Induced Lymphocytosis and Increased Cell Survival Mainly Involve the CD11b+B-Lymphocyte Subset in Sheep

Abstract : In this study, we show that bovine leukemia virus (BLV)-induced persistent lymphocytosis (PL) results from the in vivo expansion of the CD11b+ B-lymphocyte population. This subset shares phenotypic characteristics with murine and human B-1 cells. BLV interactions with the sheep B-1-like subset were explored. We found that B-1- and B-2-like cells are initially infected to similar extents. However, in long-term-infected sheep, the viral load is higher in B-1-like cells and only B-1- and not B-2-like cells show increased ex vivo survival compared to that in uninfected sheep. Ex vivo viral expression was found in both B-1- and B-2-like cells, indicating that both cell types support viral replication. Finally, cycloheximide and a protein kinase C inhibitor (H7) that blocks the ex vivo activation of viral expression did not affect the increased survival in B-1-like cells, suggesting that resistance to apoptosis is acquired in vivo. Collectively, these results indicate a peculiar susceptibility of sheep B-1-like cells to BLV transforming effects and further support the involvement of increased survival in BLV pathogenesis.
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https://hal.inrae.fr/hal-02915162
Contributor : Isabelle Schwartz <>
Submitted on : Thursday, August 13, 2020 - 5:59:08 PM
Last modification on : Tuesday, May 11, 2021 - 11:36:24 AM

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Nathalie Chevallier, Madeleine Berthelemy, Danielle Le Rhun, V. Laine, Daniel Lévy, et al.. Bovine Leukemia Virus-Induced Lymphocytosis and Increased Cell Survival Mainly Involve the CD11b+B-Lymphocyte Subset in Sheep. Journal of Virology, American Society for Microbiology, 1998, 72 (5), pp.4413-4420. ⟨10.1128/JVI.72.5.4413-4420.1998⟩. ⟨hal-02915162⟩

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