Skip to Main content Skip to Navigation
Journal articles

Annotated expressed sequence tags for studies of the regulation of reproductive modes in aphids

Abstract : The damaging effect of aphids to crops is largely determined by the spectacular rate of increase of populational expansion due to their parthenogenetic generations. Despite this, the molecular processes triggering the transition between the parthenogenetic and sexual phases between their annual life cycle have received little attention. Here, we describe a collection of genes from the cereal aphid Rhopalosiphum padi expressed during the switch from parthenogenetic to sexual reproduction. After cDNA cloning and sequencing, 726 expressed sequence tags (EST) were annotated. The R. padi EST collection contained a substantial number (139) of bacterial endosymbiont sequences. The majority of R. padi cDNAs encoded either unknown proteins (56%) or housekeeping polypeptides (38%). The large proportion of sequences without similarities in the databases is related to both their small size and their high GC content, corresponding probably to the presence of 5 0-unstranslated regions. Fifteen genes involved in developmental and differentiation events were identified by similarity to known genes. Some of these may be useful candidates for markers of the early steps of sexual differentiation. #
Document type :
Journal articles
Complete list of metadata

Cited literature [22 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02915291
Contributor : Denis Tagu <>
Submitted on : Friday, August 14, 2020 - 9:38:49 AM
Last modification on : Sunday, April 11, 2021 - 7:14:02 PM
Long-term archiving on: : Monday, November 30, 2020 - 7:33:27 PM

File

IBMB 2004.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Denis Tagu, N. Prunier-Leterme, F. Legeai, J.-P Gauthier, A Duclert, et al.. Annotated expressed sequence tags for studies of the regulation of reproductive modes in aphids. Insect Biochemistry and Molecular Biology, Elsevier, 2004, 34, pp.809 - 822. ⟨10.1016/j.ibmb.2004.05.001⟩. ⟨hal-02915291⟩

Share

Metrics

Record views

15

Files downloads

41