Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles mSystems Year : 2020

Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus

Baudoin Delépine
  • Function : Author
  • PersonId : 1078815
Marc Carnicer
  • Function : Author
  • PersonId : 976267
Cláudia Vicente
Stephanie Heux

Abstract

Bacillus methanolicus MGA3 is a thermotolerant and relatively fast-growing methylotroph able to secrete large quantities of glutamate and lysine. These natural characteristics make B. methanolicus a good candidate to become a new industrial chassis organism, especially in a methanol-based economy. Intriguingly, the only substrates known to support B. methanolicus growth as sole sources of carbon and energy are methanol, mannitol, and, to a lesser extent, glucose and arabitol. Because fluxomics provides the most direct readout of the cellular phenotype, we hypothesized that comparing methylotrophic and nonmethylotrophic metabolic states at the flux level would yield new insights into MGA3 metabolism. In this study, we designed and performed a C-13 metabolic flux analysis (C-13-MFA) of the facultative methylotroph B. methanolicus MGA3 growing on methanol, mannitol, and arabitol to compare the associated metabolic states. On methanol, results showed a greater flux in the ribulose monophosphate (RuMP) pathway than in the tricarboxylic acid (TCA) cycle, thus validating previous findings on the methylotrophy of B. methanolicus. New insights related to the utilization of cyclic RuMP versus linear dissimilation pathways and between the RuMP variants were generated. Importantly, we demonstrated that the linear detoxification pathways and the malic enzyme shared with the pentose phosphate pathway have an important role in cofactor regeneration. Finally, we identified, for the first time, the metabolic pathway used to assimilate arabitol. Overall, those data provide a better understanding of this strain under various environmental conditions.
Fichier principal
Vignette du fichier
2020_Del%C3%A9pine_mSystems-5-e00745-20_arabitol%20and%20mannitol%20and%20methanol%20flux.pdf (980.62 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Licence : CC BY - Attribution

Dates and versions

hal-02960832 , version 1 (13-12-2023)

Licence

Attribution

Identifiers

Cite

Baudoin Delépine, Marina Gil López, Marc Carnicer, Cláudia Vicente, Volker Wendisch, et al.. Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus. mSystems, 2020, 5 (5), ⟨10.1128/mSystems.00745-20⟩. ⟨hal-02960832⟩
28 View
3 Download

Altmetric

Share

Gmail Facebook X LinkedIn More