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Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting

Abstract : Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
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https://hal.inrae.fr/hal-03115175
Contributor : Nathalie Katy Chanteloup <>
Submitted on : Tuesday, January 19, 2021 - 2:24:38 PM
Last modification on : Thursday, May 6, 2021 - 2:19:51 PM

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Marion Rambault, Rachana Borkute, Emilie Doz-Deblauwe, Yves Le-Vern, Nathalie Winter, et al.. Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting. Sven Brandau; Anca Dorhoi. Myeloid-Derived Suppressor Cells, 2236, Humana Press, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1060-2_16⟩. ⟨hal-03115175⟩

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