Proton exchange effects on transverse relaxation rate were studied at low and moderate magnetic fields. Analysis was conducted on low-concentrate simple sugar (fructose and glucose) solutions modeling the vacuolar liquid in fruits. Simulated data obtained from Carver and Richards equations were used to analyze the effects of temperature and pH on parameters involved in the chemical exchange mechanisms. The results have highlighted that measurement conditions and tissue characteristics (echo time, magnetic field strength, temperature, pH, etc.) significantly impact the transverse relaxation rate via chemical exchange, even for low and mid magnetic fields and the narrow echo time ranges of TD-NMR and MRI measurements. This explains some of the divergent relaxation data from plant tissue NMR measurements reported in the literature and emphasizes the importance of taking experimental conditions and tissue characteristics into account when interpreting results. It also clearly demonstrates that the appropriate choice of experimental conditions can emphasize the effects of sugar concentration or pH variations on transverse relaxation in vacuolar liquids, making it possible to monitor these parameters accurately via transverse relaxation measurements. The impact of concentration, solute type, pH and temperature on transverse relaxation of sugar solutions were demonstrated experimentally at 1.5 T with an MRI scanner. These data were interpreted using the Carver-Richards model which was effective in estimating parameters involved in chemical exchange mechanism in the imaging experiment. (C) 2020 Elsevier Inc. All rights reserved.