NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Molecules Year : 2021

NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax

Serge Pilard

Abstract

Lignans, phenolic plant secondary metabolites, are derived from the phenylpropanoid biosynthetic pathway. Although, being investigated for their health benefits in terms of antioxidant, antitumor, anti-inflammatory and antiviral properties, the role of these molecules in plants remains incompletely elucidated; a potential role in stress response mechanisms has been, however, proposed. In this study, a non-targeted metabolomic analysis of the roots, stems, and leaves of wild-type and PLR1-RNAi transgenic flax, devoid of (+) secoisolariciresinol diglucoside ((+) SDG)-the main flaxseed lignan, was performed using H-1-NMR and LC-MS, in order to obtain further insight into the involvement of lignan in the response of plant to osmotic stress. Results showed that wild-type and lignan-deficient flax plants have different metabolic responses after being exposed to osmotic stress conditions, but they both showed the capacity to induce an adaptive response to osmotic stress. These findings suggest the indirect involvement of lignans in osmotic stress response.
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hal-03139628 , version 1 (12-11-2021)

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Kamar Hamade, Ophelie Fliniaux, Jean-Xavier J.-X. Fontaine, Roland Molinié, Elvis Otogo Nnang, et al.. NMR and LC-MS-Based Metabolomics to Study Osmotic Stress in Lignan-Deficient Flax. Molecules, 2021, 26 (3), pp.767. ⟨10.3390/molecules26030767⟩. ⟨hal-03139628⟩
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