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Etude de l'impact et choix de l'enrichissement du milieu sur l'élevage de lignées de souris transgéniques exempts d'organismes pathogènes spécifiques (EOPS) et opportunistes

Abstract : Following changes in European animal testing regulations, animal welfare has taken an important place in animal testing. These changes have also impacted the breeding conditions of laboratory animals, particularly for mice. These conditions for breeding laboratory animals are also based on the 3Rs rule (Reduce, Replace and Refine). In this last R of refinement, the improvement of animal welfare in breeding conditions requires the implementation of enrichments adapted to the mouse line. This study therefore consisted in observations to compare different means of environmental enrichment on seven specific organism pathogen free (SOPF) mouse lines at different physiological stages and different housing conditions. The mice, regardless of age and with the same genetic background C57Bl/6, were grouped into six lots and three types of accommodation. For three consecutive weeks, daily observations were made on the use of fortification, such as igloo, Kraft curls and absorbent paper. A notation of the observations was therefore made based on the aggressiveness of the mice, the use of enrichment and the shape of the nest. Slight variations in the use of enrichment and mouse behaviour were observed between weeks, with improvement over time. All the enrichments were therefore used by the different mouse lines and increasingly over time, such as improving the shape of the nest after three weeks. The most commonly enrichment used is the absorbent paper sheet, followed by Kraft curl. These observations also show that enrichment is a dependent "lineage" and depends on the age and sex of the mice. In conclusion, mice showed an increased interest in the use of appropriate enrichments. This refinement work has therefore made it possible to define, for each SOPF mouse line, the type of enrichment of the environment most adapted to the conditions of mouse breeding and their wellbeing. These observations therefore made it possible to respond as effectively as possible to the regulations in force.
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https://hal.inrae.fr/hal-03149508
Contributor : Nathalie Katy Chanteloup <>
Submitted on : Tuesday, February 23, 2021 - 10:07:01 AM
Last modification on : Monday, April 26, 2021 - 12:00:03 PM

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Corinne Beauge, Mickael Riou. Etude de l'impact et choix de l'enrichissement du milieu sur l'élevage de lignées de souris transgéniques exempts d'organismes pathogènes spécifiques (EOPS) et opportunistes. STAL Sciences et Techniques de l'Animal de Laboratoire, 2020, 48, pp.42-52. ⟨hal-03149508⟩

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