Skip to Main content Skip to Navigation
Journal articles

Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry

Abstract : Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries. The degree of methylesterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility. This work focuses on the successful online coupling of charge transfer dissociation−mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins. This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode. Compared to the UHPLC with collision-induced dissociation mass pectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results. The developed UHPLC-CTD-MS method resulted in abundant cross-ring cleavagesand especially 1,4Xn, 1,5Xn, and 2,4Xn ionswhich helped to identify most of the isomers. The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone. The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.
Complete list of metadata

https://hal.inrae.fr/hal-03150399
Contributor : Olivier Dupre <>
Submitted on : Tuesday, February 23, 2021 - 5:29:13 PM
Last modification on : Wednesday, May 19, 2021 - 10:51:50 AM

Identifiers

Collections

Citation

Praneeth Mendis, David Ropartz, Hélène Rogniaux, Glen P. Jackson, Zachary Sasiene. Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry. Analytical Chemistry, American Chemical Society, 2021, 93 (5), pp.2838-2847. ⟨10.1021/acs.analchem.0c04142⟩. ⟨hal-03150399⟩

Share

Metrics

Record views

17