Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of Experimental Botany Year : 2020

Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis

Qijian Wang
  • Function : Author
Xiaoni Zhang
  • Function : Author
Shengnan Lin
  • Function : Author
Shaozong Yang
  • Function : Author
Xiuli Yan
  • Function : Author
Manzhu Bao
  • Function : Author
Xiaopeng Fu
  • Function : Author

Abstract

The double flower is a highly important breeding trait that affects the ornamental value in many flowering plants. To get a better understanding of the genetic mechanism of double flower formation in Dianthus chinensis, we have constructed a high-density genetic map using 140 F-2 progenies derived from a cross between a single flower genotype and a double flower genotype. The linkage map was constructed using double-digest restriction site-associated DNA sequencing (ddRAD-seq) with 2353 single nucleotide polymorphisms (SNPs). Quantitative trait locus (QTL) mapping analysis was conducted for 12 horticultural traits, and major QTLs were identified for nine of the 12 traits. Among them, two major QTLs accounted for 20.7% and 78.1% of the total petal number variation, respectively. Bulked segregant RNA-seq (BSR-seq) was performed to search accurately for candidate genes associated with the double flower trait. Integrative analysis of QTL mapping and BSR-seq analysis using the reference genome of Dianthus caryophyllus suggested that an SNP mutation in the miR172 cleavage site of the A-class flower organ identity gene APETALA2 (DcAP2L) is responsible for double flower formation in Dianthus through regulating the expression of DcAG genes.
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hal-03153162 , version 1 (07-06-2021)

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Qijian Wang, Xiaoni Zhang, Shengnan Lin, Shaozong Yang, Xiuli Yan, et al.. Mapping a double flower phenotype-associated gene DcAP2L in Dianthus chinensis. Journal of Experimental Botany, 2020, 71 (6), pp.1915-1927. ⟨10.1093/jxb/erz558⟩. ⟨hal-03153162⟩
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