Increasing landscape heterogeneity, both in terms of composition and configuration, can promote natural enemies and biological control in agricultural landscapes. However, relatively poor information exists about the effects of landscape heterogeneity on lacewings, which are a major group of predators. Furthermore, temporal changes of landscape effects on natural enemy dynamics remain largely unexplored. Here, we investigated how landscape composition and configuration affect lacewings and their biological control potential on leafhoppers. Lacewings and leafhoppers were sampled from April to July in 10 vineyards located in southwestern France. The vineyards were selected along a gradient of a proportion of semi-natural habitats in the landscape. The proportion of semi-natural habitats positively affected the abundance of adults and eggs, as well as species richness, of lacewings, alone or in interaction with the sampling month. Landscape configuration was never found to enhance abundance or species richness of lacewings. Finally, the predator-prey ratio increased through time but did not respond to landscape composition or configuration. Our study highlights that the proportion of semi-natural habitats increases both abundance and diversity of lacewings in vineyard landscapes but that this effect varies over time. This result indicates the need to assess the variability of landscape effects over time to maximize biological pest control services in agricultural landscapes.