Skip to Main content Skip to Navigation
Journal articles

Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation

Abstract : Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]–Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03207081
Contributor : Christine Riou <>
Submitted on : Friday, April 23, 2021 - 9:01:42 PM
Last modification on : Friday, June 4, 2021 - 2:40:20 PM

File

JID-2021.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Juan Carlos Espinosa, Alba Marín-Moreno, Patricia Aguilar-Calvo, Sylvie Benestad, Olivier Andreoletti, et al.. Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation. Journal of Infectious Diseases, Oxford University Press (OUP), 2021, 223 (6), pp.1103-1112. ⟨10.1093/infdis/jiz646⟩. ⟨hal-03207081⟩

Share

Metrics

Record views

32

Files downloads

4