Recalcitrance of lignocellulose is caused by its structural and chemical complexity. Predicting its transformation, in particular enzymatic hydrolysis of lignocellulose into monosaccharides, is very challenging and also critical for optimizing production of biofuels then building versatile and economically viable biorefineries. Until now, many different features controlling recalcitrance have been highlighted but many studies are controversy and measurement of most of these features requires time-consuming lab analysis. We have investigated new spectral, chemical and physical features and have tested them on various biomass species with different pretreatments to see how they could be correlated to saccharification yield.