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A novel and sensitive real-time PCR system for universal detection of poxviruses

Abstract : Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects.
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https://hal.inrae.fr/hal-03213790
Contributor : Christine Riou <>
Submitted on : Friday, April 30, 2021 - 3:59:29 PM
Last modification on : Friday, June 4, 2021 - 2:40:24 PM

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Léa Luciani, Lucia Inchauste, Olivier Ferraris, Rémi Charrel, Antoine Nougairède, et al.. A novel and sensitive real-time PCR system for universal detection of poxviruses. Scientific Reports, Nature Publishing Group, 2021, 11 (1), pp.1798. ⟨10.1038/s41598-021-81376-4⟩. ⟨hal-03213790⟩

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