Skip to Main content Skip to Navigation
Journal articles

Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola

Abstract : Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii "LiuBa-8" (LB) and susceptible V. vinifera "Pinot Noir" (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H 2 O 2 , and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03236282
Contributor : Marion Desailly <>
Submitted on : Wednesday, May 26, 2021 - 11:01:13 AM
Last modification on : Thursday, May 27, 2021 - 3:09:08 AM

File

2021_Liu_HR.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Guo-Tian Liu, Bian-Bian Wang, David Lecourieux, Mei-Jie Li, Ming-Bo Liu, et al.. Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola. Horticulture research, Nature Publishing Group, 2021, 8 (1), pp.1-21. ⟨10.1038/s41438-021-00533-y⟩. ⟨hal-03236282⟩

Share

Metrics

Record views

11

Files downloads

6