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Phyllosphere Colonization by a Soil Streptomyces sp. Promotes Plant Defense Responses Against Fungal Infection

Sophie Vergnes 1 Damien Gayrard 1, 2 Marine Veyssière 1 Justine Toulotte 1 Yves Martinez 3 Valérie Dumont 4 Olivier Bouchez 5 Thomas Rey 1, 2 Bernard Dumas 1, *
* Corresponding author
3 Plateforme imagerie de Microscopie Électronique [Institut Cochin]
IC UM3 (UMR 8104 / U1016) - Institut Cochin
5 GeT-PlaGe - Génome et Transcriptome - Plateforme Génomique
GET - Plateforme Génome & Transcriptome, INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : US 1426
Abstract : Streptomycetes are soil-dwelling, filamentous actinobacteria and represent a prominent bacterial clade inside the plant root microbiota. The ability of streptomycetes to produce a broad spectrum of antifungal metabolites suggests that these bacteria could be used to manage plant diseases. Here, we describe the identification of a soil Streptomyces strain named AgN23 which strongly activates a large array of defense responses when applied on Arabidopsis thaliana leaves. AgN23 increased the biosynthesis of salicylic acid, leading to the development of salicylic acid induction deficient 2 (SID2)-dependent necrotic lesions. Size exclusion fractionation of plant elicitors secreted by AgN23 showed that these signals are tethered into high molecular weight complexes. AgN23 mycelium was able to colonize the leaf surface, leading to plant resistance against Alternaria brassicicola infection in wild-type Arabidopsis plants. AgN23-induced resistance was found partially compromised in salicylate, jasmonate, and ethylene mutants. Our data show that Streptomyces soil bacteria can develop at the surface of plant leaves to induce defense responses and protection against foliar fungal pathogens, extending their potential use to manage plant diseases.
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https://hal.inrae.fr/hal-03257978
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Submitted on : Friday, June 11, 2021 - 11:25:13 AM
Last modification on : Thursday, September 16, 2021 - 3:03:55 PM

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Sophie Vergnes, Damien Gayrard, Marine Veyssière, Justine Toulotte, Yves Martinez, et al.. Phyllosphere Colonization by a Soil Streptomyces sp. Promotes Plant Defense Responses Against Fungal Infection. Molecular Plant-Microbe Interactions, American Phytopathological Society, 2020, 33 (2), pp.223-234. ⟨10.1094/MPMI-05-19-0142-R⟩. ⟨hal-03257978⟩

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