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A Yarrowia lipolytica Strain Engineered for Pyomelanin Production

Abstract : The yeast Yarrowia lipolytica naturally produces pyomelanin. This pigment accumulates in the extracellular environment following the autoxidation and polymerization of homogentisic acid, a metabolite derived from aromatic amino acids. In this study, we used a chassis strain optimized to produce aromatic amino acids for the de novo overproduction of pyomelanin. The gene 4HPPD, which encodes an enzyme involved in homogentisic acid synthesis (4-hydroxyphenylpyruvic acid dioxygenase), was characterized and overexpressed in the chassis strain with up to three copies, leading to pyomelanin yields of 4.5 g/L. Homogentisic acid is derived from tyrosine. When engineered strains were grown in a phenylalanine-supplemented medium, pyomelanin production increased, revealing that the yeast could convert phenylalanine to tyrosine, or that the homogentisic acid pathway is strongly induced by phenylalanine.
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Contributor : Djamila Onesime <>
Submitted on : Wednesday, June 30, 2021 - 8:21:02 AM
Last modification on : Monday, July 12, 2021 - 6:08:03 PM

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Macarena Larroude, Djamila Onésime, Olivier Rué, Jean-Marc Nicaud, Tristan Rossignol. A Yarrowia lipolytica Strain Engineered for Pyomelanin Production. Microorganisms, MDPI, 2021, 9 (4), pp.838. ⟨10.3390/microorganisms9040838⟩. ⟨hal-03274344⟩



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