In drinking water production from groundwaters, biological rapid sand filters can be used for ammonium and manganese removal in aerobic conditions. However, in some boreholes, a start-up duration of several months is required to reach the required removal capacity, leading to significant water losses. Moreover, in specific industrial cases no exogenous biomass under the form of backwash water or activated sludge can be added to accelerate the process, and different approaches are seldom considered in literature. With the aim of saving water, start-up strategies coupling water temperature increase and substrate dosing were studied to accelerate the installation of biological activities, in a pilot plant fed with borehole water. These set-ups enabled a substantial acceleration of nitrification but no improvement of manganese oxidation in the experimental conditions, although the experiments showed no clear negative influence of nitrification, through nitrite accumulation, on biological manganese oxidation. To further save energy and reduce water loss, outlet water recirculation at a rate of 75% during the start-up phase was validated. The proposed start-up strategy enabled the complete installation of active biofilms with a mean start-up time reduction of 36% and water use reduction of 84% compared to the reference natural conditions.