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Article Dans Une Revue Journal of Assisted Reproduction and Genetics Année : 2021

Semi-automated versus manual embryo vitrification: inter-operator variability, time-saving, and clinical outcomes

Résumé

Purpose: Does semi-automated vitrification have lower inter-operator variability and better clinical outcomes than manual vitrification? Methods: Retrospective analyses of 282 patients whose embryos had been cryopreserved, manually with Irvine®-CBS® (MV) or semi-automatically vitrified with the GAVI® method (AV) (from November 2017 to September 2020). Both techniques were performed during the same period by 5 operators. Inter-operator variability was statistically analyzed between operators who performed the vitrification and those who performed the warming process to compare the intact survival rate (% embryos with 100% intact blastomeres) and the positive survival rate (at least 50% intact blastomeres). Additionally, the complete vitrification time was assessed for the 2 techniques according to the number of vitrified embryos. Results: Manual vitrification involved warming 338 embryos in 266 cycles for 181 couples compared to 212 embryos in 162 AV cycles for 101 patients. The positive survival rate was higher (p < 0.05) after MV (96%; 323/338) than after AV (90%; 191/212). The intact survival rate (86 vs 84%) and the clinical pregnancy rate (27 vs 22%) were not significantly different between MV and AV. Regarding the inter-operator variability, no significant difference in positive and intact survival rate was evident between the 5 technicians, neither by vitrification nor by warming steps with MV and AV. Concerning time-saving, the MV technique proved to be quicker than AV (minus 11 ± 9 min). Conclusions: Manual vitrification exhibited favorable total survival rates and was more time efficient, while both MV and AV cooling and warming treatments showed little operator variability.

Dates et versions

hal-03498151 , version 1 (20-12-2021)

Identifiants

Citer

Nicolas Gatimel, Jessika Moreau, Célia Bettiol, Jean Parinaud, Roger Léandri. Semi-automated versus manual embryo vitrification: inter-operator variability, time-saving, and clinical outcomes. Journal of Assisted Reproduction and Genetics, 2021, 38 (12), pp.3213-3222. ⟨10.1007/s10815-021-02346-3⟩. ⟨hal-03498151⟩
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