The recent decades have witnessed a significant increase in the population in peri-urban areas which led to a progressive transformation of peri-urban landscapes, and the reduced ability of agriculture to provide ecosystem services. In order to understand the complex relationships established in peri-urban areas between reference urban centre, urban services (US) and ecosystem services (ES), with particular attention to the landscape, a Discrete Choice Experiment (DCE) was carried out in the transitional peri-urban areas of six municipalities located near the city of Perugia (Italy). The two main goals of this study are analysing the effect of the presence of US and ES on the demand for housing, and exploring the implications in terms of peri-urban land use policy. The results highlight that the availability of some ES can have a significant impact on choice of housing location.