In the present study, an analysis of antimicrobial activity; and an assessment of genomic biocontrol attributes of the recently described radioresistant strain Kocuria rhizophila PT10 were conducted. PT10, a Gram-positive and yellow pigmented actinobacterial strain, was isolated from the roots of xerophyte Panicum turgidum collected from Ksar Ghilane in the south of Tunisia and its genome was sequenced. In order to assess the potential capacity of this strain to adapt to its environment, a genomic and functional characterization of key enzymes involved in polysaccharides/protein degradation, chelation of iron and production of secondary metabolites was done and revealed an interesting potential. Precisely, Cazy, antiSMASH and BAGEL analyses of the genome showed the potential of K. rhizophila PT10 to synthesize specialized metabolites (bacteriocin, linocin M18, terpenes, siderophores, etc.) and enzymes (amylase, chitinase, protease, etc.). PT10 also possesses genes potentially involved in the biosynthesis of molecules with antifungal and antimicrobial activities, such as bacilysin and cycloserine. Biocontrol assays were thus done and showed an effective antagonism under in vitro conditions against phytopathogenic fungi, Botrytis cinerea BC21 and Fusarium graminearum g1, with percents of growth inhibition of 98 and 42%, respectively. Strain PT10 also showed moderate antibacterial activity against the Gram+ Staphylococcus pasteuri PT2 and the Gram- Acinetobacter baumannii PT6. These results suggest a multivariate mode of antagonistic activity of strain PT10 against microbial pathogens through the production of hydrolytic enzymes, secondary metabolites, siderophores and other antimicrobial molecules, the characterization of which is underway.