Optimization of large-scale purification of omega gliadins and other wheat gliadins - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Journal of Cereal Science Année : 2022

Optimization of large-scale purification of omega gliadins and other wheat gliadins

Résumé

Among wheat storage proteins, omega-gliadins display a singular amino acid sequence only composed by re-petitive sequences. They have been described as a major wheat allergen and also contain T-cell epitopesimplicated in coeliac disease. To study the structural and biophysical properties of omega-gliadins and othergliadins (e.g., gluten network formation, allergic response), highly purified and concentrated fractions areneeded. In the present work, we used chromatography media screening to improve their fractionation. A SCeramic Hyper D (Pall) resin was selected for its ability to concentrate omega-gliadins in the first elution peaks.Gamma- and beta-gliadins were then separated by hydrophobic interaction chromatography and eluted with anethanol gradient. Alpha-gliadins were purified by gel filtration. Each fraction was characterized by electro-phoresis and reverse phase HPLC. The developed preparative protocol enabled the purification of several gramsof highly purified gliadins to the detriment of the yield.
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hal-03540918 , version 1 (05-01-2024)

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Véronique Solé-Jamault, Joëlle Davy, Rémy Cochereau, Adeline Boire, Colette Larré, et al.. Optimization of large-scale purification of omega gliadins and other wheat gliadins. Journal of Cereal Science, 2022, 103, pp.103386. ⟨10.1016/j.jcs.2021.103386⟩. ⟨hal-03540918⟩

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