Volume 67 of Advances in Botanical Research "Metabolomics coming of age with its technological diversity" edited by Dominique Rolin (series Editors Jean-Pierre Jacquot and Pierre Gadal) was published in 2013 and featured 13 chapters including more than 40 authors with a range of expertise in biology, biochemistry, analytical chemistry, chemometrics and bioinformatics. This volume highlighted the technological diversity of metabolomics approaches that have revolutionized life sciences, more particularly plant biology, providing a deeper understanding of the multifaceted chemical and physiological variety of plant systems. Since its initiation, metabolomics has never failed to deliver further progress in research fields. In this anniversary volume 100 of ABR, a special focus is placed on the contribution of metabolomics to the study of dietary supplements, where Chemistry, Botanics and Physiology cross path. We also highlight the current challenges and future perspectives in this inspiring, fast-evolving area, which holds great promise for unraveling novel mechanisms by which food supplements benefit Human nutrition and health.