A highly conserved core bacterial microbiota with nitrogen-fixation capacity inhabits the xylem sap in maize plants - Archive ouverte HAL Access content directly
Journal Articles Nature Communications Year : 2022

A highly conserved core bacterial microbiota with nitrogen-fixation capacity inhabits the xylem sap in maize plants

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Liyu Zhang
  • Function : Author
Meiling Zhang
  • Function : Author
Shuyu Huang
  • Function : Author
Lujun Li
  • Function : Author
Qiang Gao
  • Function : Author
Yin Wang
  • Function : Author
Shuiqing Zhang
  • Function : Author
Shaomin Huang
  • Function : Author
Liang Yuan
  • Function : Author
Yanchen Wen
  • Function : Author
Kailou Liu
  • Function : Author
Xichu Yu
  • Function : Author
Dongchu Li
  • Function : Author
Lu Zhang
  • Function : Author
Xinpeng Xu
  • Function : Author
Hailei Wei
  • Function : Author
Ping He
Wei Zhou
  • Function : Author
Laurent Philippot
  • Function : Author
  • PersonId : 1144377
Chao Ai

Abstract

Abstract Microbiomes are important for crop performance. However, a deeper knowledge of crop-associated microbial communities is needed to harness beneficial host-microbe interactions. Here, by assessing the assembly and functions of maize microbiomes across soil types, climate zones, and genotypes, we found that the stem xylem selectively recruits highly conserved microbes dominated by Gammaproteobacteria. We showed that the proportion of bacterial taxa carrying the nitrogenase gene ( nifH ) was larger in stem xylem than in other organs such as root and leaf endosphere. Of the 25 core bacterial taxa identified in xylem sap, several isolated strains were confirmed to be active nitrogen-fixers or to assist with biological nitrogen fixation. On this basis, we established synthetic communities (SynComs) consisting of two core diazotrophs and two helpers. GFP-tagged strains and 15 N isotopic dilution method demonstrated that these SynComs do thrive and contribute, through biological nitrogen fixation, 11.8% of the total N accumulated in maize stems. These core taxa in xylem sap represent an untapped resource that can be exploited to increase crop productivity.

Dates and versions

hal-03716540 , version 1 (07-07-2022)

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Liyu Zhang, Meiling Zhang, Shuyu Huang, Lujun Li, Qiang Gao, et al.. A highly conserved core bacterial microbiota with nitrogen-fixation capacity inhabits the xylem sap in maize plants. Nature Communications, 2022, 13 (1), pp.3361. ⟨10.1038/s41467-022-31113-w⟩. ⟨hal-03716540⟩
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