Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA - Archive ouverte HAL Access content directly
Journal Articles Journal of Virology Year : 2003

Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA

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Nicolás Molina
  • Function : Author
José Ignacio Núñez
  • Function : Author
Francisco Sobrino
  • Function : Author
Margarita Sáiz
  • Function : Author

Abstract

ABSTRACT We assayed the infectivity of naked foot-and-mouth disease virus (FMDV) RNA by direct inoculation of suckling mice. Our results demonstrate that transcripts generated from full-length cDNA clones were infectious, as was virion-extracted RNA. Interestingly, infectious virus could be recovered from a mutant transcript encoding amino acid substitution L-147→P in capsid protein VP1, known to be noninfectious for BHK-21 cells. The model described here provides a useful tool for virulence studies in vivo, bypassing possible selection of variants during viral replication in cell culture.

Dates and versions

hal-03716765 , version 1 (07-07-2022)

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Eric Baranowski, Nicolás Molina, José Ignacio Núñez, Francisco Sobrino, Margarita Sáiz. Recovery of Infectious Foot-and-Mouth Disease Virus from Suckling Mice after Direct Inoculation with In Vitro-Transcribed RNA. Journal of Virology, 2003, 77 (20), pp.11290-11295. ⟨10.1128/JVI.77.20.11290-11295.2003⟩. ⟨hal-03716765⟩

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