Mutations in DMI3 and SUNN Modify the Appressorium-Responsive Root Proteome in Arbuscular Mycorrhiza - Archive ouverte HAL Access content directly
Journal Articles Molecular Plant-Microbe Interactions Year : 2006

Mutations in DMI3 and SUNN Modify the Appressorium-Responsive Root Proteome in Arbuscular Mycorrhiza

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1
Nardjis Amiour
  • Function : Author
Franck Robert
  • Function : Author
Silvio Gianinazzi
  • Function : Author
Eliane Dumas-Gaudot
  • Function : Author

Abstract

Modification of the Medicago truncatula root proteome during the early stage of arbuscular mycorrhizal symbiosis was investigated by comparing, using two-dimensional electrophoresis, the protein patterns obtained from non-inoculated roots and roots synchronized for Glomus intraradices appressorium formation. This approach was conducted in wild-type (J5), mycorrhiza-defective (TRV25, dmi3), and autoregulation-defective (TR122, sunn) M. truncatula genotypes. The groups of proteins that responded to appressorium formation were further compared between wild-type and mutant genotypes; few overlaps and major differences were recorded, demonstrating that mutations in DMI3 and SUNN modified the appressorium-responsive root proteome. Except for a chalcone reductase, none of the differentially displayed proteins that could be identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry previously was known as appressorium responsive. A DMI3-dependent increased accumulation of signal transduction-related proteins (dehydroascorbate reductase, cyclophilin, and actin depolymerization factor) was found to precede mycorrhiza establishment. Differences in the accumulation of proteins related to plant defense reactions, cytoskeleton rearrangements, and auxin signaling upon symbiont contact were recorded between wild-type and hypermycorrhizal genotypes, pointing to some putative pathways by which SUNN may regulate very early arbuscule formation.

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hal-03744674 , version 1 (03-08-2022)

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Nardjis Amiour, Ghislaine G. Recorbet, Franck Robert, Silvio Gianinazzi, Eliane Dumas-Gaudot. Mutations in DMI3 and SUNN Modify the Appressorium-Responsive Root Proteome in Arbuscular Mycorrhiza. Molecular Plant-Microbe Interactions, 2006, 19 (9), pp.988-997. ⟨10.1094/MPMI-19-0988⟩. ⟨hal-03744674⟩
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