Identification of two novel putative satellite RNAs with hammerhead structures in the virome of French and Spanish carrot samples
Résumé
Carrot virome analysis using high-throughput sequencing revealed the presence of two RNA molecules with properties of satellite RNAs and homologous to the satellite RNA of cereal yellow dwarf virus-RPV (CYDV-RPV). Satellite 1 is 298 nt long, while Satellite 2 is 368 nt long. Their positive and negative genome strands contain hammerhead ribozymes similar to those found in other self-cleaving satellite RNAs. While both satellites were detected in Spanish carrot populations, only satellite 2 was found in French carrot populations. The most likely helper virus for these two satellites is carrot red leaf virus (CtRLV), which similar to CYDV-RPV is a Polerovirus.
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